Along with lncap and pc3 cells, du145 cells were once considered part of the triad that constituted the gold standard of pca cell culture lines. Pc3 pc3 is a human prostate cancer cell line used in prostate cancer research and drug development. John masters ucl was grown in ksfm supplemented with 10% fbs, 0. The lncap cancer cell line was established from a human lymph node metastatic lesion of prostatic adenocarcinoma. Lipogenic signalling modulates prostate cancer cell.
The xenografts models nude mice are used for testing the tumorigenicity and metastatic ability of cancer cell lines. Cosmic, the catalogue of somatic mutations in cancer, is the worlds largest and most comprehensive resource for exploring the impact of somatic mutations in human cancer. Integrative proteomic and phosphoproteomic profiling of. Effects of radiation on prostate cancer cells lines. Gene expression omnibus geo database, accession no gse4500. The bone metastasisderived pc3 and the lymph node metastasisderived lncap prostate cancer cell lines are widely studied, having been described in thousands of publications over the last four decades. Downloading rna seq data for prostate cancer cell lines.
The urologic epithelial stem cell database uesc a web. The cancer cell types included prostate cancer cell lines lncap, c42, cl1. In the first study, we will determine the cellular response of three prostate cancer cell lines pc3, du145, and 22rv1 after single or fractionated doses of radiation. Find your cell lines and models of interest in one easy search.
Hello colleagues, i need to download rna seq data for prostate cancer cell lines like du145 and prec i tried navigating through ccle, oncomine and geo but i cant seem to find rna seq data for normal untreated prostate cancer cell lines. Behavior of the biomarker in a prostate cancer cell line compendium. Abstract a database of prostate cancer proteomics has been created by using the results of a proteomic study. You did a great service to the cancer research community and by that to the patients that donated the samplesclinical pathologist, karolinska university hospital. Cancer cell line encyclopedia novartisbroad, nature 2012 1020 samples.
Rc58t cells were derived from a radical prostatectomy specimen from a 52yearold white man. The human prostate cancer cell lines pc3, du145 and 22rv1 were purchased from the american type culture collection atcc and maintained in rpmi1640 medium supplemented with 10% fetal bovine serum fbs, 50 uml penicillin50. For each cell line, gene expression in treated channel 2. One way of dealing with that heterogeneity is to test large numbers of patientderived cell lines for common vulnerabilities. As and ai cell lines are different in their transcriptional programs and degree of dna copy number alterations. To identify modulators of androgenindependent prostate cancer that could serve as potential biomarkers of aggressive disease, we performed an in depth proteomic analysis of the conditioned media of five androgenindependent prostate cancer cell lines du145, pc3, lncapsf, ppc1, and 22rv1, two androgendependent cell lines lncap and vcap. We present the utility of massively parallel sequencing by synthesis for profiling the transcriptome of a human prostate cancer cellline, lncap, that has been treated with the synthetic androgen, r1881. Deciphering the novel molecular mechanisms underlying stemlike traits may provide important insight for developing novel therapeutics. An integrated database of chemosensitivity to 55 anticancer. The adica cell lines ce series grow well in the absence of androgen. Common cell lines used in prostate cancer research. The gene expression profiles of cell lines showed limited similarities to prostate tumors harvested at surgery.
Prostate cancer is the development of cancer in the prostate, a gland in the male reproductive system. Screening of a panel of prostate cancer cell lines for parpi sensitivity in order to identify prostate cancer cell lines sensitive to parpi treatment, we performed a screening with a panel of available cell lines cultured under ngc. Sobel re, sadar md 2005 cell lines used in prostate cancer research. The human normal prostate epithelial cell line rwpe1 and human pca cell lines, pc3 and lncap, were purchased from the cell bank of the chinese academy of sciences shanghai, china where they were characterized by dna fingerprinting, isozyme detection, mycoplasma detection and cell vitality detection. Immortalized benign cell lines bph1 and ep156t, as well as arpositive lncap, lapc4, vcap, ducap. Btf3 sustains cancer stemlike phenotype of prostate cancer. We consistently found tsg101 to be present at similar levels in exosomes from both cell lines and thus used this marker for quantification by stripping each respective western blot and reblotting them with a tsg101 specific antibody figure 4a. Start using cosmic by searching for a gene, cancer type, mutation, etc. Klf5 inhibits angiogenesis in pten deficient prostate cancer. Genomewide association studies identified singlenucleotide polymorphism snp rs55958994 as a significant variant associated with increased susceptibility to prostate cancer. In vitro and in vivo model systems used in prostate cancer research.
Establishment of test conditions and assessment of hormonestimulated growth and druginduced cytostatic and cytotoxic effects. Two recent proteomic studies revealed exosomal proteins related to prostate cancer. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. Aug 12, 2003 we did not find mutations in exon 2 of the klf6 gene in any of the 32 tumour samples that we examined nor in the human prostate tumour cell lines pc3, du145 and lncap. However, its role in mitochondrial metabolism remains unclear. After 6 passages as monolayers, these cells senesced. Lineage relationship of prostate cancer cell types based on gene. Pc3 cells are useful in investigating biochemical changes in advanced prostate cancer cells and in assessing their response to chemotherapeutic agents. We present the utility of massively parallel sequencing by synthesis for profiling the transcriptome of a human prostate cancer cell line, lncap, that has been treated with the synthetic androgen, r1881. Scientists have established a few prostate cancer cell lines to investigate the mechanism involved in the progression of prostate cancer. Affymetrix array analysis of prostate cancer cell lines. Odyssey imager, and analyzed using the provided licor software. Characterisation and manipulation of docetaxel resistant.
The ability of the 51gene biomarker to identify chemicals that modulate ar was examined in a compendium of biosets derived from prostate cancer cell lines assembled as described in materials and methods section. This microarray dataset accompanies the publication by kim et al, the retinoic acid synthesis gene aldh1a2 is a candidate tumor suppressor in prostate cancer. They both reported 266 and 220 proteins, which is similar to the. The human prostate cancer cell lines pc3, du145, lncap, 22rv1, and vcap. Identification of key pathways and genes in pten mutation. Networkbased analysis of prostate cancer cell lines. Networkbased analysis of prostate cancer cell lines reveals novel. Mutation analysis of 24 known cancer genes in the nci60 cell. Author summary prostate cancer cell lines represent an important model system to. Jan 20, 2020 researchers tested approximately 4,518 drug compounds on 578 human cancer cell lines and found nearly 50 that have previously unrecognized anti cancer activity.
Apr 11, 2012 in addition, genistein cooperates with vorinostat to induce cell death in prostate cancer cell lines with a greater effect on early stage prostate cancer. Properties of common prostate cancer cell lines pc3 pc3 is a human prostate cancer cell line used in prostate cancer research and drug development. Atcc stands ready to support our customers needs during the coronavirus pandemic. The nci60 cell lines were assembled by the national cancer institute as an in vitro anticancer drug screen, which went into operation in 1990. Dna methyltransferase inhibitor 5azadctreated prostate. The importance of cancer cell lines as in vitro models in cancer methylome analysis and anticancer drugs testing 143 tumour. The information and materials data made available on this web site. Mouse prostate cancer cell lines established from primary. If you have prostate cancer or are close to someone who does, knowing what to expect can help you cope. The cell line name and lineage are listed at the top of the cell line page. Adapt identifies an escrt complex composition that. An overview of publicly available patientcentered prostate. Prostate cancer is the most common cancer among men after skin cancer, but it can often be treated successfully.
All information is derived from the bc cancer agency prostate cancer cell line database cited previously unless otherwise specified. The student will gather and analyse the masses of genetic data available from prostate cancer patients biopsy and blood samples to work out which treatments, and which clinical trials, might work best for him. The heterogeneity of human cancers is a major obstacle to developing therapies. Here you can find out all about prostate cancer, including risk factors, symptoms, how it is found, and how it is treated. In vitro and in vivo model systems used in prostate cancer. The panel comprises 60 human cancer cell lines representing nine tissue of origin types. Analysis of the prostate cancer cell line lncap transcriptome. Olaparib is effective in combination with, and as maintenance. Wholegenome sequence of the metastatic pc3 and lncap. Steap2 knockdown reduces the invasive potential of. B prostate cancer progression over time, from localized asymptomatic castrationsensitive to metastatic castrationresistant disease. In this study, we show that this variant is located in an enhancer active in prostate cancer cells. Sep 16, 20 fastgrowing cancer cell lines are indicated with a yellow dot and slowergrowing cell lines are indicated by a red dot. Pten phosphatase and tensin homolog mutant is the top common mutated genes in prostate cancer, which makes it a promising biomarker in future individualized treatment.
Proteomic profiling of androgenindependent prostate cancer cell. Gpnmboa protein increases the invasiveness of human metastatic prostate cancer cell lines du145 and pc3 through mmp2 and mmp9 activity. By resequencing the mrna products of a cell, one may rapidly discover polymorphisms and splice variants particular to that cell. This project is divided into two distinct studies relative to prostate cancer. This cell line was introduced in 1997 and was the result of a series of subcutaneous xenografting experiments into scid mice 55. Author summary prostate cancer cell lines represent an important model system to characterize molecular alterations that contribute to radioresistance, but irradiation can cause deletions and amplifications of dna segments that affect hundreds of genes.
This sample is limited in amount and the genetic manipulation is almost impossible 3. Many specialized cellline databases have been established over the years that cater to a specific taxonomic range or a category of cell lines. The remaining cell lines had an intermediate growth rate. Genomic analysis of dna repair genes and androgen signaling in.
The 1542cptx primary prostate cancer cell line kindly gifted by prof. Name source metastasis in mice doubling time psa rna psa protein ar rna ar protein cytokeratin expression. The human prostate cancer cell lines pc3, du145, lncap, 22rv1, and vcap and the normal prostate epithelial cell line rwpe1 were purchased from the american type culture collection atcc, manassas, va. The ubiquitin ligase ube4a inhibits prostate cancer progression by targeting interleukinlike emt inducer ilei. The ar splice variant ar v567es is constitutively active to investigate the function of ar v567es in both benign and cancer prostate cells, we cotransfected the p69 benign immortalized prostate epithelial cell line and the arnull m12 human prostate cancer cell line, with the ar activity reporter construct pgl3probasin arearr3luciferase arr3luc and a construct expressing ar v567es. The mutation data table displays a list of mutations specifics to the cell line or gene. We generated rnaseq data from multiple prostate cancer lines and used. The need for in vivo models was an outgrowth of the understanding that cancer cell lines in general and prostate lines in particular do not and cannot recapitulate the disease processes that occur in living tissues. I am looking for a dataset with data gathered from african and african caribbean men while undergoing tests for prostate cancer.
Prostate cancer pca is the second most common cancer worldwide, with one in eight men being diagnosed in the uk and one in fivesix in the. This dataset provides a context for the use of prostate cancer cell lines as models for clinical cancers. So is it good to analyze the prostate cancer cell lines pc3 and du145 or lncap also and then arrive at a. Prostate cancer models cell lines, xenografts, pdx, io. Prostate cancer models cell lines, xenografts, pdx, io models. Rwpe1 and wpestem are two prostate cell lines which. Building a database of prostate cancer genetic information. The dataset comprises four prostate cancer cell lines treated with the dna methyltransferase inhibitor 5azadc. Among american men, prostate cancer is the most common, noncutaneous malignancy that accounted for an estimated 241,000 new cases and 34,000 deaths in 2011. Atcc normal human primary prostate epithelial cells, when grown in prostate epithelial cell basal media supplemented with prostate epithelial cell growth kit components, provide an ideal cell system to propagate prostate epithelial cells in serumfree conditions. The cancer cells may spread from the prostate to other areas of the body, particularly the bones and lymph nodes.
The alva41 cell line was derived from a bony metastasis from a human prostatic carcinoma by drs. Dna copy number changes in prostate cancer cell lines omicx. Fullyvalidated and characterized prostate cancer in vitro cell lines, in vivo xenografts and pdx for preclinical drug. To view a cell lines information, search for it from the front page. Jan 20, 2010 the adca cell lines e2 and e4 and the adica cell lines ce1 and ce2 display biallelic deletion at the pten gene locus, an event which is specific for the prostate epithelium for this mouse model and a fairly similar level of expression of the androgen receptor ar. Mouse prostate cancer cell lines established from primary and. Using this software, pathway analysis was performed, obtaining. All transcriptome datasets are available on our public database uesc 19. Identification of androgen receptor modulators in a. Grp78 modulates cell adhesion markers in prostate cancer and. Alterations in master genes lead to mitochondrial metabolic rewiring and provide an appealing target to inhibit cancer progression and improve survival. Analysis of human prostate cancers and cell lines for.
Prostate cancer pca remains one of the leading adult malignancies. C silac cell line culture preparation, spikein and mass spectrometry analysis of the proteomes and phosphoproteomes. Proteomic profiling of androgenindependent prostate cancer. The adca cell lines e2 and e4 and the adica cell lines ce1 and ce2 display biallelic deletion at the pten gene locus, an event which is specific for the prostate epithelium for this mouse model and a fairly similar level of expression of the androgen receptor ar. Cpdr multicenter national database prostate cancer research. This in combination with the small number of cell lines that are usually considered does not allow a straightforward identification of.
Many studies on pca have been carried out, each taking much time before the data is collected and ready to be analyzed. An overview of publicly available patientcentered prostate cancer datasets prostate cancer pca is the second most common cancer in men, and the second leading cause of death from cancer in men. Du145 and pc3 human prostate cancer cell lines express. Using a panel of 64 cell lines to represent various cell populations in different organs and tissues of the human body, the mrna expression of all human genes are characterized by deep rnasequencing. Proteomic profiling of androgenindependent prostate.
Using appropriate cell lines and cell line derived xenograft models with genetic features of interest can enhance your early stage in vitro and in vivo drug discovery programs. However, the mechanisms by which this snp mediates increased risk to cancer are still unknown. The center for prostate disease research cpdr multicenter national database program seeks to longitudinally collect comprehensive demographic, clinical, treatment and quality of life outcomes data to learn more about prostate disease, particularly prostate cancer, in an effort to better prevent, diagnose and treat patients. These studies examined gene expression profiles in 60 cell lines that were pharmacologically characterized on treatment with various. Dec 18, 2018 ncad expression levels transcription and protein were evaluated upon sirna mediated silencing of grp78 in the mm. The molecular signatures database hallmark gene set collection. For one such study, ras initiative researchers constructed gfplabeled derivatives of dozens of cell lines that express mutant kras. The second study focuses on identifying radioresistanct biomarkers of prostate cancer.
Epigenetic and genetic features of 24 colon cancer cell lines. Neuroendocrine prostate cancer multiinstitute, nat med 2016. The cell atlas provides highresolution insights into the expression and spatiotemporal distribution of proteins within human cells. Prostate cancer prostate cancer information and overview. Cell lines immortalized by exposure to human telomerase reverse transcriptase rc58t and rc58thtert. Our database, comprising both gene expression data and drug activity data for the same set of cell lines, was first developed by ross et al. Lncap, pc3, and du145 are commonly used prostate cancer cell lines. Metastatic castrationsensitive prostate cancer msk, clin cancer res 2020 424 samples. Immunohistochemistry and immunofluorescence assays in prostatic tissues. We obtained gene expression data of prostate cancer from tcga the cancer genome atlas database for analysis. The prostate cancer risk variant rs55958994 regulates. In the latest genomewide copy number change studies, klf5s deep deletion, which likely indicates homozygous deletion, has been detected in 10% of prostate cancers in the cancer genome atlas tcga database tcgadata. Cultured cancer cell lines, such as the humanderived pc3 and lncap, are critical for.
In later stages, it can lead to difficulty urinating, blood in. To view a cell line s information, search for it from the front page. Crownbio has made searching for cell lines and xenografts easy using xeno base, our collated online database worlds first online cell line and xenograft. Teasing apart molecular interactions and alterations in cancer cells is of incredible worth, but they do not take into account. Researchers tested approximately 4,518 drug compounds on 578 human cancer cell lines and found nearly 50 that have previously unrecognized anti cancer. Tsg101 is a component of the escrt machinery and an established exosomal marker in prostate cancer. Cellminer is a webaccessible relational database tool that organizes, navigates, and integrates multiple types of molecular profile data on the nci60 cell line panel at the t dna, rna, protein, and smallmolecule levels. Because du145 and pc3 prostate cancer cell lines are considered to be arnegative,, our observations that. May 28, 2019 cancer stemlike traits contribute to prostate cancer pca progression and metastasis.
Metabolic rewiring is a common feature of many cancer types, including prostate cancer pca. Pc3 cells are also used to study viral infection in mammalian cells that exhibit an immune response. Furthermore, we evaluated the effects of grp78 knockdown kd on epithelialmesenchymal emt transition markers, morphological changes and adhesion of pc3 cells. Moreover, prc1 activity correlated with emt and stemness traits in dnpc patient samples and metastatic cell lines, suggesting that prc1 may sustain the oncogenicity of prostate cancer cells that have acquired mesenchymal and stemlike transcriptional traits in support of metastatic capacity. Kinase pim1 promotes prostate cancer cell growth via cmyc. In particular the dataset should have patient information such age. Ostenson, american lake veterans affairs medical center, tacoma, washington.
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